Recombinant human TYRP1 protein

TYRP1, also known as 5, 6-dihydroxyindole-2-carboxylic acid oxidase, is a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. It is a melanocyte-specific gene product involved in eumelanin synthesis. It is involved in the oxidation of 5, 6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5, 6-quinone-2-carboxylic acid. It is regulated by the microphthalmia-associated transcription factor (MITF) . Recombinant human TYRP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

5, 6-dihydroxyindole-2-carboxylic acid oxidase, TYRP1, b-PROTEIN, CAS2, CATB, GP75, OCA3, TRP, TRP1, TYRP

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

52.5 kDa (462aa)

Additionnal Information

TYRP1, ATGP3435-10 µg, ATGP3435-20 µg, ATGP3435-50 µg, ATGP3435-100 µg, ATGP3435-250 µg, ATGP3435-500 µg, ATGP3435-1 mg, ATGP3435-10, ATGP3435-20, ATGP3435-50, ATGP3435-100, ATGP3435-250, ATGP3435-500, ATGP3435-1

References & Citations

Sarangarajan R., et al. (2001) Pigment Cell Res. 14:437-444.; ; Chalupa A., et al. (2015) J Invest Dermatol. 135:202-211.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_000541

Uniprot Accession Number

P17643

Species

Human

N Terminal Sequence

Ala

AA Sequence

QFPRQCA TVEALRSGMC CPDLSPVSGP GTDRCGSSSG RGRCEAVTAD SRPHSPQYPH DGRDDREVWP LRFFNRTCHC NGNFSGHNCG TCRPGWRGAA CDQRVLIVRR NLLDLSKEEK NHFVRALDMA KRTTHPLFVI ATRRSEEILG PDGNTPQFEN ISIYNYFVWT HYYSVKKTFL GVGQESFGEV DFSHEGPAFL TWHRYHLLRL EKDMQEMLQE PSFSLPYWNF ATGKNVCDIC TDDLMGSRSN FDSTLISPNS VFSQWRVVCD SLEDYDTLGT LCNSTEDGPI RRNPAGNVAR PMVQRLPEPQ DVAQCLEVGL FDTPPFYSNS TNSFRNTVEG YSDPTGKYDP AVRSLHNLAH LFLNGTGGQT HLSPNDPIFV LLHTFTDAVF DEWLRRYNAD ISTFPLENAP IGHNRQYNMV PFWPPVTNTE MFVTAPDNLG YTYEIQWPSR EFSVPE