Recombinant human Guanylate kinase 1/GUK1 protein

GUK1, also known as GMK, belongs to the guanylate kinase family. This protein exists as a monomer that catalyzes the ATP-dependent conversion of GMP to GDP, thereby playing an essential role in the recycling of GMP. Via its catalytic activity, GUK1 is thought to participate in regulating the supply of guanine nucleotides to signal transduction pathways. Overexpression of GUK1 is associated with pituitary adenocarcinomas, suggesting that GUK1 is involved in tumorigenesis. Recombinant human GUK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >100 units/mg and is defined as the amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH.

Product Name Alternative

Guanylate kinase, GMK, GMP kinase, GMPK

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is >100unit/mg and is defined as the amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH.

Molecular Weight

23.9 kDa (217aa) confirmed by MALDI-TOF

Additionnal Information

Guanylate Kinase, Guanylate kinase, GMK, GMP kinase, ATGP3432-10 µg, ATGP3432-20 µg, ATGP3432-50 µg, ATGP3432-100 µg, ATGP3432-250 µg, ATGP3432-500 µg, ATGP3432-1 mg, ATGP3432-10, ATGP3432-20, ATGP3432-50, ATGP3432-100, ATGP3432-250, ATGP3432-500, ATGP3432-1

References & Citations

Da Rocha AA., et al. (2006) Pituitary. 9 (2) :83-92. ; ; Brady WA., et al. (1996) J Biol Chem. 271 (28) :16734-40

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl

Scientific Category

Cancer

NCBI Accession Number

NP_000849

Uniprot Accession Number

Q16774

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA