Recombinant human Glutamine synthetase/GLUL protein
Glutamine synthetase (GLUL), which is therefore able to regulate intracellular concentrations of glutamate. GLUL catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. GLUL is essential for proliferation of fetal skin fibroblasts and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in GLUL are associated with congenital glutamine deficiency. Recombinant GLUL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Glutamine synthase, Glutamate-ammonia ligase, GLNS, Glutamate-ammonia ligase, GS, Palmitoyltransferase GLUL
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
44.2 kDa (393aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Vermeulen T., et al, (2008) Arch Biochem Biophys. 478:96-102; ; Liaw SH., et al. (1995) Protein Sci. 4 (11) : 2358-65.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 200 mM NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001028216
Uniprot Accession Number
P15104
Species
Human
AA Sequence
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