Recombinant human Monoglyceride Lipase/MGLL protein

MGLL is membrane-associated member of the serine hydrolase superfamily. MGLL functions together with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides. It is most abundantly expressed in skeletal muscle and adipose tissue. Recombinant human MGLL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is >170 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C.

Product Name Alternative

Monoglyceride lipase, Hu-K5, HuK5, MAGL, MGL, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Bioactivity

Specific activity is >170unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C.

Molecular Weight

36.4 kDa (333aa) confirmed by MALDI-TOF

Additionnal Information

Hu K5, HuK5, Hu-K5, Lysophospholipase homolog, Lysophospholipase-like, MAGL, MGL, MGLL, Monoglyceride Lipase, ATGP3430-10 µg, ATGP3430-20 µg, ATGP3430-50 µg, ATGP3430-100 µg, ATGP3430-250 µg, ATGP3430-500 µg, ATGP3430-1 mg, ATGP3430-010, ATGP3430-020, ATGP3430-050, ATGP3430-100, ATGP3430-250, ATGP3430-500, ATGP3430-01M

References & Citations

Cravatt BF., et al. (2010) Cell. 140 (1) :49-61.; ; Lambert DM., et al (2010) Chembiochem.11 (2) :218-27.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_009214

Uniprot Accession Number

Q99685

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH METGPEDPSS MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE LARMLMGLDL LVFAHDHVGH GQSEGERMVV SDFHVFVRDV LQHVDSMQKD YPGLPVFLLG HSMGGAIAIL TAAERPGHFA GMVLISPLVL ANPESATTFK VLAAKVLNLV LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFG IQLLNAVSRV ERALPKLTVP FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTN SVFHEINMWV SQRTATAGTA SPP