Recombinant mouse PGAM1 protein

PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) . Recombinant mouse PGAM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is >150units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Product Name Alternative

Phosphoglycerate mutase 1, 2310050F24Rik, Pgam-1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is >150unit/mg, in which one unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Molecular Weight

31.4 kDa (278aa) confirmed by MALDI-TOF

Additionnal Information

PGAM1, Phosphoglycerate mutase 1, 2310050F24Rik, Pgam-1, ATGP3427-10 µg, ATGP3427-20 µg, ATGP3427-50 µg, ATGP3427-100 µg, ATGP3427-250 µg, ATGP3427-500 µg, ATGP3427-1 mg, ATGP3427-10, ATGP3427-20, ATGP3427-50, ATGP3427-100, ATGP3427-250, ATGP3427-500, ATGP3427-1

References & Citations

Cao L., et al. (2007) J. Immunol. 179:5864-5876; ; Ballif B.A., et al. (2008) J. Proteome Res. 7:311-318

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_075907

Uniprot Accession Number

Q9DBJ1

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK