Recombinant human PGAM1 protein

PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) . Mutations in this protein cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >300 units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Product Name Alternative

Phosphoglycerate mutase 1, Phosphoglycerate mutase isozyme B, PGAM-B, PGAMA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is >300unit/mg, in which one unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Molecular Weight

30.9 kDa (274aa) confirmed by MALDI-TOF

Additionnal Information

PGAM1, Phosphoglycerate mutase 1, Phosphoglycerate mutase isozyme B, PGAM-B, PGAMA, ATGP3426-10 µg, ATGP3426-20 µg, ATGP3426-50 µg, ATGP3426-100 µg, ATGP3426-250 µg, ATGP3426-500 µg, ATGP3426-1 mg, ATGP3426-10, ATGP3426-20, ATGP3426-50, ATGP3426-100, ATGP3426-250, ATGP3426-500, ATGP3426-1

References & Citations

Sakoda S., et al. (1988) J Biol Chem. 263 (32) :16899-905. ; ; Junien C., et al. (1982) Ann Genet. 25 (1) :25-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_002620.1

Uniprot Accession Number

P18669

Species

Human

AA Sequence

MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK