Recombinant human Meprin A subunit alpha/MEP1A protein

MEP1A, also known as meprin A subunit alpha, is a single-pass type I membrane protein which belongs to the peptidase M12A family. It abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. It is capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Recombinant human MEP1A, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Meprin A subunit alpha, MEP1A, PPHA, Endopeptidase-2, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha, PABA peptide hydrolase, PPH alpha

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

67.4 kDa (589aa)

Additionnal Information

Endopeptidase 2, Endopeptidase2, Endopeptidase-2, MEP1A, Meprin A subunit alpha, N benzoyl L tyrosyl P amino benzoic acid hydrolase subunit alpha, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha, PABA peptide hydrolase, PPH A, PPH alpha, PPHA, PPHalpha, ATGP3425-10 µg, ATGP3425-20 µg, ATGP3425-50 µg, ATGP3425-100 µg, ATGP3425-250 µg, ATGP3425-500 µg, ATGP3425-1 mg, ATGP3425-010, ATGP3425-020, ATGP3425-050, ATGP3425-100, ATGP3425-250, ATGP3425-500, ATGP3425-01M

References & Citations

Geurts N., et al. (2012) FEBS Lett. 586:4264-4269.; ; Minder P., et al. (2012) J Biol Chem. 287:35201-35211.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_005579

Uniprot Accession Number

Q16819

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPVPIKYLP EENVHDADFG EQKDISEINL AAGLDLFQGD ILLQKSRNGL RDPNTRWTFP IPYILADNLG LNAKGAILYA FEMFRLKSCV DFKPYEGESS YIIFQQFDGC WSEVGDQHVG QNISIGQGCA YKAIIEHEIL HALGFYHEQS RTDRDDYVNI WWDQILSGYQ HNFDTYDDSL ITDLNTPYDY ESLMHYQPFS FNKNASVPTI TAKIPEFNSI IGQRLDFSAI DLERLNRMYN CTTTHTLLDH CTFEKANICG MIQGTRDDTD WAHQDSAQAG EVDHTLLGQC TGAGYFMQFS TSSGSAEEAA LLESRILYPK RKQQCLQFFY KMTGSPSDRL VVWVRRDDST GNVRKLVKVQ TFQGDDDHNW KIAHVVLKEE QKFRYLFQGT KGDPQNSTGG IYLDDITLTE TPCPTGVWTV RNFSQVLENT SKGDKLQSPR FYNSEGYGFG VTLYPNSRES SGYLRLAFHV CSGENDAILE WPVENRQVII TILDQEPDVR NRMSSSMVFT TSKSHTSPAI NDTVIWDRPS RVGTYHTDCN CFRSIDLGWS GFISHQMLKR RSFLKNDDLI IFVDFEDITH LSQHHHHHH