Recombinant human IL-4 protein
IL4, also known as Interleukin 4 isoform1, is a pleiotropic cytokine that has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of naive helper T-cell to Th2 cells. IL4 is a co-stimulator of DNA-synthesis and is induces the expression of class II MHC molecules on resting B-cells. It also enhances both secretion and cell surface expression of IgE and IgG1. Recombinant human IL4, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IL-4 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤0.6 ng/mL.
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by BCA assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤0.6 ng/mL.
Molecular Weight
15 kDa (130aa)
Additionnal Information
References & Citations
Van Kimmenade A., et al (1988) . Eur J Biochem. 173 (1) : 109-14; ; Yokota T., et al (1986) . Proc. Nati. Acad. Sci. USA. 83 (16) : 5894-8;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000580.1
Uniprot Accession Number
P05112
Species
Human
AA Sequence
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
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