Recombinant human LTA/TNF-beta protein

LTA, also known as lymphotoxin alpha and tumor necrosis factor beta, belongs to the tumor necrosis factor family. It is highly inducible, secreted, and forms heterotrimers with lymphotoxin beta which anchor lymphotoxin alpha to the cell surface. It is expressed by activated naive CD4 cells, unpolarized IL2 secreting effectors, and Th1 effectors and mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. Recombinant human LTA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques

Product Specifications

Specifications

Human LTA/TNF-beta induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Product Name Alternative

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, TNFB, TNFSF1, TNF superfamily, member 1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 1 ng/mL.

Molecular Weight

19.7 kDa (180aa)

Additionnal Information

Lymphotoxin-a , TNF-b, Lymphotoxin alpha, LTA, LT, TNFB, TNFSF1, TNLG1E, LTalpha, DIF, LT, LT alpha, LT-alpha, Lta, Lymphotoxin alpha, Lymphotoxin alpha (TNF superfamily, member 1), Lymphotoxin-alpha, TNF B, TNF superfamily member 1, TNF-beta, TNFB, TNFB_HUMAN, TNFbeta, TNFSF1, Tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1, ATGP3420-10 µg, ATGP3420-20 µg, ATGP3420-50 µg, ATGP3420-100 µg, ATGP3420-250 µg, ATGP3420-500 µg, ATGP3420-1 mg, ATGP3420-10, ATGP3420-20, ATGP3420-50, ATGP3420-100, ATGP3420-250, ATGP3420-500, ATGP3420-1

References & Citations

Messer G., et al. (1991) J Exp Med. 173:209-219.; ; Gramaglia I., et al. (1999) J Immunol 162:1333-1338

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001153212

Uniprot Accession Number

P01374

Species

Human

N Terminal Sequence

Ala

AA Sequence

LPGVGLT PSAAQTARQH PKMHLAHSTL KPAAHLIGDP SKQNSLLWRA NTDRAFLQDG FSLSNNSLLV PTSGIYFVYS QVVFSGKAYS PKATSSPLYL AHEVQLFSSQ YPFHVPLLSS QKMVYPGLQE PWLHSMYHGA AFQLTQGDQL STHTDGIPHL VLSPSTVFFG AFAL