Recombinant human CD90/Thy1 protein
THY1, as known as Thy-1 membrane glycoprotein, is a cell membrane protein which contains 1lg-like V-type (immunoglobulin-like) domain. This protein is a member of the immunoglobulin superfamily of adhesion molecules with constitutive expression on fibroblast cells. It can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Also, this protein led to some of the initial biochemical description and characterization of a vertebrate glycophosphatidylinositol (GPI) anchor and also the first demonstration of tissue specific differential glycosylation. Recombinant human THY1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
13.3 kDa (117aa)
Additionnal Information
References & Citations
Li Q., et al. (2015) PLoS Pathog. 11:E1004999.; ; Woeller CF., et al. (2015) FASEB J. 29:920-931.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_006279.2
Uniprot Accession Number
P04216
Species
Human
N Terminal Sequence
Gln20
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