Recombinant human TNFRSF7/CD27 protein
CD27, as known as CD27 antigen, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycosylation on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD 70 on lymphocytes and dendritic cells. This protein plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. Recombinant human CD27, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human CD27 Ligand/TNFSF7 (CAT# ATGP3336) is coated at 10 µg/mL (100 ul/well) can bind Human CD27/TNFRSF7. The ED50 range ≤ 0.5 µg/mL.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 85% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human CD27 Ligand/TNFSF7 (CAT# ATGP3336) . The ED50 range ≤ 0.5 µg/mL.
Molecular Weight
46.4 kDa (413aa)
Additionnal Information
References & Citations
Clemente A., et al. (2013) Clin. Exp. Immunol. 174:97-108.; ; Huang J., et al. (2013) J. Immunol. 190:6250-6258.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_001233.1
Uniprot Accession Number
P26842
Species
Human
N Terminal Sequence
Ala
AA Sequence
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