Recombinant human TNFRSF7/CD27 protein

CD27, as known as CD27 antigen, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycosylation on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD 70 on lymphocytes and dendritic cells. This protein plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. Recombinant human CD27, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CD27 Ligand/TNFSF7 (CAT# ATGP3336) is coated at 10 µg/mL (100 ul/well) can bind Human CD27/TNFRSF7. The ED50 range ≤ 0.5 µg/mL.

Product Name Alternative

CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, TNFRSF7, T cell antivation antigen S152, TP55

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human CD27 Ligand/TNFSF7 (CAT# ATGP3336) . The ED50 range ≤ 0.5 µg/mL.

Molecular Weight

46.4 kDa (413aa)

Additionnal Information

CD27, TNFRSF7, CD27 antigen, CD27, S152, LPFS2, T14, TNFRSF7, Tp55, CD 27, CD27 antigen, CD27 molecule, CD27_HUMAN, CD27L receptor, LPFS2, MGC20393, OTTHUMP00000238557, S152, T cell activation antigen CD27, T cell antivation antigen S152, T-cell activation antigen CD27, T14, TNFRSF 7, TNFRSF7, TNFSF7, Tp 55, Tp55, Tumor necrosis factor receptor superfamily member 7, ATGP3416-10 µg, ATGP3416-20 µg, ATGP3416-50 µg, ATGP3416-100 µg, ATGP3416-250 µg, ATGP3416-500 µg, ATGP3416-1 mg, ATGP3416-10, ATGP3416-20, ATGP3416-50, ATGP3416-100, ATGP3416-250, ATGP3416-500, ATGP3416-1

References & Citations

Clemente A., et al. (2013) Clin. Exp. Immunol. 174:97-108.; ; Huang J., et al. (2013) J. Immunol. 190:6250-6258.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001233.1

Uniprot Accession Number

P26842

Species

Human

N Terminal Sequence

Ala

AA Sequence

TPAPKSC PERHYWAQGK LCCQMCEPGT FLVKDCDQHR KAAQCDPCIP GVSFSPDHHT RPHCESCRHC NSGLLVRNCT ITANAECACR NGWQCRDKEC TECDPLPNPS LTARSSQALS PHPQPTHLPY VSEMLEARTA GHMQTLADFR QLPARTLSTH WPPQRSLCSS DFIR