Recombinant human Renin protein
REN, also known as renin, is a member of the peptidase A1 family. It is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. It is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Recombinant human REN, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques
Product Specifications
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
43.3 kDa (391aa)
Additionnal Information
References & Citations
Nguyen G., et al. (2014) Hypertension. 63:297-302.; ; Nabi AH., et al. (2012) Biochem Biophys Res Commun. 428:506-511.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cardiovascular
NCBI Accession Number
NP_000528
Uniprot Accession Number
P00797
Species
Human
N Terminal Sequence
Leu24
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