Recombinant human TIMP-4 protein

TIMP4, as known as metalloproteinase inhibitor 4, is a family of secreted protein that regulates the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases. There are four known members of the family, TIMP1, 2, 3 and 4. TIMP4 is produced by a wide range of tissues, particularly brain, heart, ovary and skeletal muscle. Limited studies have shown that this protein has a tumor suppressive effect against Wilm s tumor, exhibits negative correlation with glioma malignancy and is found in breast carcinoma cells. Recombinant human TIMP4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Metalloproteinase inhibitor 4, TIMP4, TIMP-4, Metalloproteinase inhibitor 4, TIMP 4, TIMP metallopeptidase inhibitor 4, TIMP-4, Timp4, TIMP4_HUMAN, Tissue inhibitor of metalloproteinase 4, Tissue inhibitor of metalloproteinases 4

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.5 kDa (204aa)

Additionnal Information

TIMP4, Metalloproteinase inhibitor 4 , TIMP4, TIMP-4, Metalloproteinase inhibitor 4TIMP 4, TIMP metallopeptidase inhibitor 4TIMP-4, Timp4, TIMP4_HUMANTissue inhibitor of metalloproteinase 4Tissue inhibitor of metalloproteinases 4, ATGP3407-10 µg, ATGP3407-20 µg, ATGP3407-50 µg, ATGP3407-100 µg, ATGP3407-250 µg, ATGP3407-500 µg, ATGP3407-1 mg, ATGP3407-10, ATGP3407-20, ATGP3407-50, ATGP3407-100, ATGP3407-250, ATGP3407-500, ATGP3407-1

References & Citations

Greene J., et al. (1996) J Biol Chem. 271:30375-80.; ; Groft LL., et al. (2001) Br J Cancer. 85:55-63.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_003247

Uniprot Accession Number

Q99727

Species

Human

N Terminal Sequence

Ala

AA Sequence

CSCAPAH PQQHICHSAL VIRAKISSEK VVPASADPAD TEKMLRYEIK QIKMFKGFEK VKDVQYIYTP FDSSLCGVKL EANSQKQYLL TGQVLSDGKV FIHLCNYIEP WEDLSLVQRE SLNHHYHLNC GCQITTCYTV PCTISAPNEC LWTDWLLERK LYGYQAQHYV CMKHVDGTCS WYRGHLPLRK EFVDIVQP