Recombinant human TIMP-4 protein
TIMP4, as known as metalloproteinase inhibitor 4, is a family of secreted protein that regulates the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases. There are four known members of the family, TIMP1, 2, 3 and 4. TIMP4 is produced by a wide range of tissues, particularly brain, heart, ovary and skeletal muscle. Limited studies have shown that this protein has a tumor suppressive effect against Wilm s tumor, exhibits negative correlation with glioma malignancy and is found in breast carcinoma cells. Recombinant human TIMP4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
23.5 kDa (204aa)
Additionnal Information
References & Citations
Greene J., et al. (1996) J Biol Chem. 271:30375-80.; ; Groft LL., et al. (2001) Br J Cancer. 85:55-63.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cardiovascular
NCBI Accession Number
NP_003247
Uniprot Accession Number
Q99727
Species
Human
N Terminal Sequence
Ala
AA Sequence
Frequently Asked Questions
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