Recombinant human Angiopoietin-like 3/ANGPTL3 protein

ANGPTL3, as known as angiopoietin-related protein 3, is a secreted glycoprotein that is structurally related to the angiopoietins. This protein directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids. This activity requires a putative heparin-binding motif which is N-terminal to the coiled coil domain. Recombinant human ANGPTL3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

ANG-5, Angiopoietin 5, Angiopoietin-related protein 3, ANGPT5, ANGPTL3, ANL3, FHBL2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

52.9 kDa (453aa)

Additionnal Information

Angiopoietin-like3, ATGP3406-10 µg, ATGP3406-20 µg, ATGP3406-50 µg, ATGP3406-100 µg, ATGP3406-250 µg, ATGP3406-500 µg, ATGP3406-1 mg, ATGP3406-10, ATGP3406-20, ATGP3406-50, ATGP3406-100, ATGP3406-250, ATGP3406-500, ATGP3406-1

References & Citations

Tikka A., et al. (2014) Biosci. Rep. 34:E00160.; ; Ono M., et al. (2003) J. Biol. Chem. 278:41804-41809.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Scientific Category

Angiogenesis

NCBI Accession Number

NP_055310

Uniprot Accession Number

Q9Y5C1

Species

Human

N Terminal Sequence

Ala

AA Sequence

SRIDQDN SSFDSLSPEP KSRFAMLDDV KILANGLLQL GHGLKDFVHK TKGQINDIFQ LNIFDQSFY DLSLQTSEIK EEEKELRRTT YKLQVKNEEV KNMSLELNSK LESLLEEKIL LQQKVKYLEE QLTNLIQNQP ETPEHPEVTS LKTFVEKQDN SIKDLLQTVE DQYKQLNQQH SQIKEIENQL RRTSIQEPTE ISLSSKPRAP RTTPFLQLNE IRNVKHDGIP AECTTIYNRG EHTSGMYAIR PSNSQVFHVY CDVISGSPWT LIQHRIDGSQ NFNETWENYK YGFGRLDGEF WLGLEKIYSI VKQSNYVLRI ELEDWKDNKH YIEYSFYLGN HETNYTLHLV AITGNVPNAI PENKDLVFST WDHKAKGHFN CPEGYSGGWW WHDECGENNL NGKYNKPRAK SKPERRRGLS WKSQNGRLYS IKSTKMLIHP TDSESFE