Recombinant human Glutamine synthetase/GLUL protein

GLUL also known as Glutamine synthetase. It is a trimetallic enzyme containing two divalent cation sites and one monovalent cation site per subunit. GLUL is able to regulate intracellular concentrations of glutamate and catalyzes the synthesis of glutamine form glutamate and ammonia. It is ubiquitously expressed in the human and plays a major role for many metabolic pathways such as cell proliferation, inhibition of apoptosis, and cell signaling. Recombinant Human GLUL was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Specifications

Specific activity is > 2.000 pmol/min/ug, and is defined as the amount of enzyme that convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.

Product Name Alternative

Glutamine synthase, Glutamate-ammonia ligase, GLNS, Glutamate-ammonia ligase, GS, Palmitoyltransferase GLUL

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 85% by SDS-PAGE

Bioactivity

Specific activity is > 2.000pmol/min/ug, and is defined as the amount of enzyme that convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.

Molecular Weight

42 kDa (373aa) confirmed by MALDI-TOF

Additionnal Information

GLUL, Glutamine synthetase , GLNS, GS, PIG43, PIG59, cell proliferation-inducing protein 59, GLNA, GLNA_HUMAN, GLNS, GLULGlutamate ammonia ligase, Glutamate decarboxylaseGlutamate--ammonia ligase, glutamine synthaseGlutamine synthetase, GS, PIG 43, PIG 59, PIG43PIG59, Proliferation inducing protein 43, ATGP3404-10 µg, ATGP3404-20 µg, ATGP3404-50 µg, ATGP3404-100 µg, ATGP3404-250 µg, ATGP3404-500 µg, ATGP3404-1 mg, ATGP3404-10, ATGP3404-20, ATGP3404-50, ATGP3404-100, ATGP3404-250, ATGP3404-500, ATGP3404-1

References & Citations

Liaw SH., et al. (1995) Protein Sci. 4 (11) : 2358-65.; ; Vermeulen T., et al. (2008) Arch Biochem Biophys. 478 (1) : 96-102.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1mM PMSF

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001028216.1

Uniprot Accession Number

P15104

Species

Human

AA Sequence

MTTSASSHLN KGIKQVYMSL PQGEKVQAMY IWIDGTGEGL RCKTRTLDSE PKCVEELPEW NFDGSSTLQS EGSNSDMYLV PAAMFRDPFR KDPNKLVLCE VFKYNRRPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP YYCGVGADRA YGRDIVEAHY RACLYAGVKI AGTNAEVMPA QWEFQIGPCE GISMGDHLWV ARFILHRVCE DFGVIATFDP KPIPGNWNGA GCHTNFSTKA MREENGLKYI EEAIEKLSKR HQYHIRAYDP KGGLDNARRL TGFHETSNIN DFSAGVANRS ASIRIPRTVG QEKKGYFEDR RPSANCDPFS VTEALIRTCL LNETGDEPFQ YKN