Recombinant mouse EphA4 protein

Epha4, as known as ephrin type-A receptor 4, is a single-pass type 1 membrane protein which belongs to the protein kinase superfamily and ephrin receptor subfamily. This protein may play a role in a signal transduction process involved in hindbrain pattern formation. The ephrins and ephrin receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Recombinant mouse Epha4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

EPH receptor A4, Ephrin type-A receptor 4, Tyrosine-protein kinase receptor MPK-3, Tyrosine-protein kinase receptor SEK-1, Cek8, Hek8, rb, Sek, Tyro1

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

59.3 kDa (536aa)

Additionnal Information

EphA4, Ephrin type-A receptor 4 , Epha4, 2900005c20Rik, AI385584, Cek8, Hek8, rb, Sek, Sek1, Tyro1, ATGP3403-10 µg, ATGP3403-20 µg, ATGP3403-50 µg, ATGP3403-100 µg, ATGP3403-250 µg, ATGP3403-500 µg, ATGP3403-1 mg, ATGP3403-10, ATGP3403-20, ATGP3403-50, ATGP3403-100, ATGP3403-250, ATGP3403-500, ATGP3403-1

References & Citations

Borgius L., et al. (2014) J. Neurosci. 34:3841-3853.; ; Steinecke A., et al. (2014) Development 141:460-471.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_031962

Uniprot Accession Number

Q03137

Species

Mouse

N Terminal Sequence

Val20

AA Sequence

VTGSRVYPAN EVTLLDSRSV QGELGWIASP LEGGWEEVSI MDEKNTPIRT YQVCNVMEAS QNNWLRTDWI TREGAQRVYI EIKFTLRDCN SLPGVMGTCK ETFNLYYYES DNDKERFIRE SQFGKIDTIA ADESFTQVDI GDRIMKLNTE IRDVGPLSKK GFYLAFQDVG ACIALVSVRV FYKKCPLTVR NLAQFPDTIT GADTSSLVEV RGSCVNNSEE KDVPKMYCGA DGEWLVPIGN CLCNAGHEEQ NGECQACKIG YYKALSTDAS CAKCPPHSYS VWEGATSCTC DRGFFRADND AASMPCTRPP SAPLNLISNV NETSVNLEWS SPQNTGGRQD ISYNVVCKKC GAGDPSKCRP CGSGVHYTPQ QNGLKTTRVS ITDLLAHTNY TFEIWAVNGV SKYNPSPDQS VSVTVTTNQA APSSIALVQA KEVTRYSVAL AWLEPDRPNG VILEYEVKYY EKDQNERSYR IVRTAARNTD IKGLNPLTSY VFHVRARTAA GYGDFSEPLE VTTNTVPSRI IGDGANSTLE HHHHHH