Recombinant human CD229/SLAMF3 protein

LY9, as known as T-lymphocyte surface antigen Ly-9 isoform, is a type 1 transmembrane glycoprotein in the SLAM subgroup of the CD2 family. This protein consists of an extracellular domain with two lg-like V-set and two lg-like truncated C2-set domains. It is expressed on T and B cells, thymocytes, and more weakly on NK cells. Homophilic binding between LY9 molecules is mediated by the N-terminal lg-like domain. Also, this protein knockout mice show minimally impaired immune responses, suggesting functional redundancy with other molecules. Recombinant human LY9, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

T-lymphocyte surface antigen Ly-9 isoform, LY9, CD229, hly9, mLY9, SLAMF3, CD229 Cell surface molecule Ly-9, Cell surface molecule Ly9, hly9, LY 9, Ly9, LY9_HUMAN, Lymphocyte antigen 9, mLY9, Signaling lymphocytic activation molecule 3, SLAM family member 3, SLAMF3, T lymphocyte surface antigen Ly9 precursor, T-lymphocyte surface antigen Ly-9

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

45.9 kDa (416aa)

Additionnal Information

CD229, SLAMF3, T-lymphocyte surface antigen Ly-9 isoform , LY9, CD229, hly9, mLY9, SLAMF3, CD229Cell surface molecule Ly-9, Cell surface molecule Ly9hly9, LY 9, Ly9, LY9_HUMAN, Lymphocyte antigen 9mLY9, Signaling lymphocytic activation molecule 3SLAM family member 3, SLAMF3, T lymphocyte surface antigen Ly9 precursor, T-lymphocyte surface antigen Ly-9, ATGP3400-10 µg, ATGP3400-20 µg, ATGP3400-50 µg, ATGP3400-100 µg, ATGP3400-250 µg, ATGP3400-500 µg, ATGP3400-1 mg, ATGP3400-10, ATGP3400-20, ATGP3400-50, ATGP3400-100, ATGP3400-250, ATGP3400-500, ATGP3400-1

References & Citations

Hagberg N., et al. (2013) J. Immunol. 191:2989-2998.; ; Chatterjee M., et al. (2012) J. Biol. Chem. 287:38168-38177.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_002339

Uniprot Accession Number

Q9HBG7

Species

Human

N Terminal Sequence

Ala

AA Sequence

KDSAPTV VSGILGGSVT LPLNISVDTE IENVIWIGPK NALAFARPKE NVTIMVKSYL GRLDITKWSY SLCISNLTLN DAGSYKAQIN QRNFEVTTEE EFTLFVYEQL QEPQVTMKSV KVSENFSCNI TLMCSVKGAE KSVLYSWTPR EPHASESNGG SILTVSRTPC DPDLPYICTA QNPVSQRSSL PVHVGQFCTD PGASRGGTTG ETVVGVLGEP VTLPLALPAC RDTEKVVWLF NTSIISKERE EAATADPLIK SRDPYKNRVW VSSQDCSLKI SQLKIEDAGP YHAYVCSEAS SVTSMTHVTL LIYRRLRKPK ITWSLRHSED GICRISLTCS VEDGGNTVMY TWTPLQKEAV VSQGESHLNV SWRSSENHPN LTCTASNPVS RSSHQFLSEN ICSGPERNTK