Recombinant mouse IL-1 beta/IL1B protein
IL-1beta is an inflammatory cytokine that might modulate angiogenesis by directly interacting with vascular endothelial cells or enhancing the production of proangiogenic factors via paracrine control. IL-1beta stimulates endothelial cell migration and proliferation, adhesion-molecule expression, inflammatory mediator production, and leukocyte recruitment. IL-1beta is required for tumor growth, metastasis, and angiogenesis in several animal models. Recombinant mouse IL-1beta, fused to His tag, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse IL-1 beta /IL-1F2 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ≤0.1 ng/mL.
Product Name Alternative
Interleukin-1 beta, IL-1 beta
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤0.1 ng/mL.
Molecular Weight
21 kDa (189aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Saijo Y, et al, (2002) J. Immunol. 169:469-475.; ; Torisu H, et al, (2000) J. Cancer. 85:182-188.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_032387
Uniprot Accession Number
P10749
Species
Mouse
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
