Recombinant mouse IL-1 beta/IL1B protein

IL-1beta is an inflammatory cytokine that might modulate angiogenesis by directly interacting with vascular endothelial cells or enhancing the production of proangiogenic factors via paracrine control. IL-1beta stimulates endothelial cell migration and proliferation, adhesion-molecule expression, inflammatory mediator production, and leukocyte recruitment. IL-1beta is required for tumor growth, metastasis, and angiogenesis in several animal models. Recombinant mouse IL-1beta, fused to His tag, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse IL-1 beta /IL-1F2 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ≤0.1 ng/mL.

Product Name Alternative

Interleukin-1 beta, IL-1 beta

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤0.1 ng/mL.

Molecular Weight

21 kDa (189aa) confirmed by MALDI-TOF

Additionnal Information

Pro interleukin 1 beta, Preinterleukin beta, Preinterleukin 1 beta, OTTHUMP00000162031, Osteoclast activating factor, OAF, Mouse IL1 beta, LAF, Interleukin-1 beta, Interleukin 1 beta precursor, Interleukin 1 beta Catabolin, Interleukin 1 beta, IL1F2, IL-1beta, IL1B_HUMAN, IL-1b, IL1B, IL1 F2, IL-1 beta, IL1 BETA, IL1 b, IL 1B, IL 1 beta, IL 1, IFN beta inducing factor, Hematopoietin 1, H1, Catabolin, ATGP3398-10 µg, ATGP3398-20 µg, ATGP3398-50 µg, ATGP3398-100 µg, ATGP3398-250 µg, ATGP3398-500 µg, ATGP3398-1 mg, ATGP3398-010, ATGP3398-020, ATGP3398-050, ATGP3398-100, ATGP3398-250, ATGP3398-500, ATGP3398-01M

References & Citations

Saijo Y, et al, (2002) J. Immunol. 169:469-475.; ; Torisu H, et al, (2000) J. Cancer. 85:182-188.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_032387

Uniprot Accession Number

P10749

Species

Mouse

AA Sequence

MVPI RQLHYRLRDE QQKSLVLSDP YELKALHLNG QNINQQVIFS MSFVQGEPSN DKIPVALGLK GKNLYLSCVM KDGTPTLQLE SVDPKQYPKK KMEKRFVFNK IEVKSKVEFE SAEFPNWYIS TSQAEHKPVF LGNNSGQDII DFTMESVSS