Recombinant human Guanine deaminase/GDA protein

GDA, also known as guanine deaminase, catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human GDA, was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 2,000 pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.

Product Name Alternative

Guanine deaminase isoform b, Guanase, Guanine aminase, Guanine aminohydrolase, GAH, p51-nedasin, CYPIN, Cytoplasmic PSD-95 interactor

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 2,000pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.

Molecular Weight

51 kDa (454aa) confirmed by MALDI-TOF

Additionnal Information

GDA, Guanine deaminase isoform b , CYPIN, GUANASE, NEDASIN, CYPIN, Cytoplasmic PSD95 interactor, D10S105E, GAH, GDAGUAD_HUMAN, Guanase, Guanine aminase, Guanine aminohydrolase, Guanine deaminase, MGC9982, NEDASIN, p51-nedasin, SLC25A16, ATGP3394-10 µg, ATGP3394-20 µg, ATGP3394-50 µg, ATGP3394-100 µg, ATGP3394-250 µg, ATGP3394-500 µg, ATGP3394-1 mg, ATGP3394-10, ATGP3394-20, ATGP3394-50, ATGP3394-100, ATGP3394-250, ATGP3394-500, ATGP3394-1

References & Citations

Gang Yuan, James C. Bin, et al. (1999) . J Biol. Chem. 274 (12) :8175-80; ; Trautwein-Schult A, et al (2014) J Mol Microbiol Biotechnol. 24 (2) :67-81;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004284

Uniprot Accession Number

Q9Y2T3

Species

Human

AA Sequence

MCAAQMPPLA HIFRGTFVHS TWTCPMEVLR DHLLGVSDSG KIVFLEEASQ QEKLAKEWCF KPCEIRELSH HEFFMPGLVD THIHASQYSF AGSSIDLPLL EWLTKYTFPA EHRFQNIDFA EEVYTRVVRR TLKNGTTTAC YFATIHTDSS LLLADITDKF GQRAFVGKVC MDLNDTFPEY KETTEESIKE TERFVSEMLQ KNYSRVKPIV TPRFSLSCSE TLMGELGNIA KTRDLHIQSH ISENRDEVEA VKNLYPSYKN YTSVYDKNNL LTNKTVMAHG CYLSAEELNV FHERGASIAH CPNSNLSLSS GFLNVLEVLK HEVKIGLGTD VAGGYSYSML DAIRRAVMVS NILLINKVNE KSLTLKEVFR LATLGGSQAL GLDGEIGNFE VGKEFDAILI NPKASDSPID LFYGDFFGDI SEAVIQKFLY LGDDRNIEEV YVGGKQVVPF SSSV