Recombinant human Prostatic Acid Phosphatase/ACPP protein
ACPP, also known as prostatic acid phosphatase isoform PAP, is a type I integral membrane protein of the plasma membrane and lysosomes, and a secreted form also exists. The concentration of ACPP is elevated in the circulation of prostate cancer patients, making the enzyme a marker for the progression of prostate cancer. Recombinant human ACPP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
41.8 kDa (360aa)
Additionnal Information
References & Citations
Chuang TD., et al. (2010) J Biol Chem. 285:23598-23606.; ; Taira A., et al. (2007) Oncology. 21:1003-1010.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_001090.2
Uniprot Accession Number
P15309
Species
Human
N Terminal Sequence
Lys33
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