Recombinant human CD30/TNFRSF8 protein

TNFRSF8, also known as tumor necrosis factor receptor superfamily member 8, is a cell membrane protein of the tumor necrosis factor receptor superfamily and is expressed by activated T and B cells but not resting. It contributes to thymic negative selection by inducing the apoptotic cell death of CD4+CD8+ T cells. It can regulate proliferation of lymphocytes and may also play an important role in human immunodeficiency virus replication. Recombinant human TNFRSF8, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CD30 Ligand/TNFSF8 (CAT# ATGP3343) is coated at 2 µg/mL (100 ul/well) can bind Human CD30/TNFRSF8. The ED50 range ≤ 100 ng/mL.

Product Name Alternative

Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30, D1S166E

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human CD30 Ligand/TNFSF8 (CAT# ATGP3343) . The ED50 range ≤ 100 ng/mL.

Molecular Weight

66.7 kDa (613aa)

Additionnal Information

CD30, TNFRSF8, Tumor necrosis factor receptor superfamily member 8, TNFRSF8, CD30, D1S166E, Ki-1, CD 30, CD30 antigen, CD30L receptor, Cytokine receptor CD30, D1S166E, KI 1, KI 1 antigen, Ki-1 antigen, KI1, Lymphocyte activation antigen CD30, TNFRSF 8, Tnfrsf8, TNR8_HUMAN, Tumor necrosis factor receptor superfamily member 8, ATGP3389-10 µg, ATGP3389-20 µg, ATGP3389-50 µg, ATGP3389-100 µg, ATGP3389-250 µg, ATGP3389-500 µg, ATGP3389-1 mg, ATGP3389-10, ATGP3389-20, ATGP3389-50, ATGP3389-100, ATGP3389-250, ATGP3389-500, ATGP3389-1

References & Citations

Kennedy MK., et al. (2006) Immunology 118:143-152.; ; Shanebeck SD., et al. (1995) Eur J Immunol. 25:2147-2153.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001234

Uniprot Accession Number

P28908

Species

Human

N Terminal Sequence

Ala

AA Sequence

FPQDRPF EDTCHGNPSH YYDKAVRRCC YRCPMGLFPT QQCPQRPTDC RKQCEPDYYL DEADRCTACV TCSRDDLVEK TPCAWNSSRV CECRPGMFCS TSAVNSCARC FFHSVCPAGM IVKFPGTAQK NTVCEPASPG VSPACASPEN CKEPSSGTIP QAKPTPVSPA TSSASTMPVR GGTRLAQEAA SKLTRAPDSP SSVGRPSSDP GLSPTQPCPE GSGDCRKQCE PDYYLDEAGR CTACVSCSRD DLVEKTPCAW NSSRTCECRP GMICATSATN SCARCVPYPI CAAETVTKPQ DMAEKDTTFE APPLGTQPDC NPTPENGEAP ASTSPTQSLL VDSQASKTLP IPTSAPVALS STGK