Recombinant human Creatine kinase B/CKB protein
CKB, also known as Creatine Kinase B-type, is encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. Recombinant human CKB, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, CPK-B, B-CK, CKBB
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
42.6 kDa (381aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Steen C., et al. (2010) PLoS One. 5 (5) :e10811.; ; Hara H., et al. (2009) J Virol. 83 (10) 5137-47.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_001814
Uniprot Accession Number
P12277
Species
Human
AA Sequence
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