Recombinant human EphA2 protein

EPHA2, also known as ephrin type-A receptor 2, is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. It is implicated as positional labels that may guide the development of neural topographic maps. It has also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization. Recombinant human EPHA2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques

Product Specifications

Specifications

Human EphA2 is coated at 2 µg/mL (100 ul/well) can bind Human Ephrin-A1 (CAT# ATGP3782) in a Functional ELISA assay.

Product Name Alternative

EPH receptor A2, Ephrin type-A receptor 2, EPHA2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, ARCC2, CTPA, CTPP1, CTRCT6

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human Ephrin-A1 (CAT# ATGP3782) .

Molecular Weight

57.3 kDa (520aa)

Additionnal Information

EphA2, Ephrin type-A receptor 2, EPHA2, ARCC2, CTPA, CTPP1, CTRCT6, ECK, AW545284, EC 2.7.10.1, Eck , Eph receptor A2, EPHA2, EPHA2_HUMAN, Ephrin receptor, Ephrin receptor EphA2, Ephrin type A receptor 2, Ephrin type-A receptor 2, Epithelial cell kinase, Epithelial cell receptor protein tyrosine kinase, Myk 2, Myk2, Sek 2Sek2, Tyrosine protein kinase receptor ECK, Tyrosine-protein kinase receptor ECK, Tyrosine-protein kinase receptor MPK-5, Tyrosine-protein kinase receptor SEK-2, ATGP3378-10 µg, ATGP3378-20 µg, ATGP3378-50 µg, ATGP3378-100 µg, ATGP3378-250 µg, ATGP3378-500 µg, ATGP3378-1 mg, ATGP3378-10, ATGP3378-20, ATGP3378-50, ATGP3378-100, ATGP3378-250, ATGP3378-500, ATGP3378-1

References & Citations

Flanagan JG., et al. (1998) Annu Rev Neurosci. 21:309-345.; ; Cheng N., et al. (2002) Cytokine Growth Factor Rev. 13:75-85.

Other References

Kim SM et al., Developing CAR-T/NK cells that target EphA2 for non-small cell lung cancer treatment. (Front Immunol. 2025) {https://pubmed.ncbi.nlm.nih.gov/40181964/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_004422.2

Uniprot Accession Number

P29317

Species

Human

N Terminal Sequence

Ala

AA Sequence

KEVVLLD FAAAGGELGW LTHPYGKGWD LMQNIMNDMP IYMYSVCNVM SGDQDNWLRT NWVYRGEAER IFIELKFTVR DCNSFPGGAS SCKETFNLYY AESDLDYGTN FQKRLFTKID TIAPDEITVS SDFEARHVKL NVEERSVGPL TRKGFYLAFQ DIGACVALLS VRVYYKKCPE LLQGLAHFPE TIAGSDAPSL ATVAGTCVDH AVVPPGGEEP RMHCAVDGEW LVPIGQCLCQ AGYEKVEDAC QACSPGFFKF EASESPCLEC PEHTLPSPEG ATSCECEEGF FRAPQDPASM PCTRPPSAPH YLTAVGMGAK VELRWTPPQD SGGREDIVYS VTCEQCWPES GECGPCEASV RYSEPPHGLT RTSVTVSDLE PHMNYTFTVE ARNGVSGLVT SRSFRTASVS INQTEPPKVR LEGRSTTSLS VSWSIPPPQQ SRVWKYEVTY RKKGDSNSYN VRRTEGFSVT LDDLAPDTTY LVQVQALTQE GQGAGSKVHE FQTLSPEGSG NLAV