Recombinant human S100A8 protein
S100A8, also known as S100A8 isoform d. S100A8 is a member of the S100 family, EF-hand superfamily of Calcium binding proteins. S100A8 protein is localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Altered expression of this protein is associated with the disease cystic fibrosis. Recombinant human S100A8, fused to His-tag at N-terminus, fused to Myc-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8
Expression System
E.coli
Antigen Species
Human
Tag
His-Myc-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
14.5 kDa (127aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kerkhoff C., et al. (1998) Biochim. Biophys. 1448 (2) :200-11. ; ; Nacken W., et al. (2003) Microsc Res Tech. 60 (6) :569-80.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl
Scientific Category
Cancer
NCBI Accession Number
NP_002955
Uniprot Accession Number
P05109
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMLTELE KALNSIIDVY HKYSLIKGNF HAVYRDDLKK LLETECPQYI RKKGADVWFK ELDINTDGAV NFQEFLILVI KMGVAAHKKS HEESHKEEQK LISEEDL
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
