Recombinant rat Lactate Dehydrogenase A/LDHA protein
Ldha, also known as lactate dehydrogenase A, catalyzes the conversion of pyruvate to lactate, utilizing NADH as a cofactor. It has been identified as a potential therapeutic target in the area of cancer metabolism. Reduction in Ldha activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. Recombinant rat Ldha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Product Name Alternative
LDH muscle subunit, LDH-M
Expression System
Baculovirus
Antigen Species
Rat
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 200unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Molecular Weight
37.5 kDa (340aa)
Additionnal Information
References & Citations
Ward RA., et al. (2012) J Med Chem. 55:3285-3306.; ; Fantin VR., et al. (2006) Cancer Cell. 9:425-434.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 20% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_058721
Uniprot Accession Number
P04642
Species
Rat
N Terminal Sequence
Met1
AA Sequence
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