What sizes are available for Recombinant rat Lactate Dehydrogenase A/LDHA protein?

Recombinant rat Lactate Dehydrogenase A/LDHA protein is available in the following size(s): 10 µg.

Recombinant rat Lactate Dehydrogenase A/LDHA protein

Q: What is the catalog number for Recombinant rat Lactate Dehydrogenase A/LDHA protein?

The catalog number for Recombinant rat Lactate Dehydrogenase A/LDHA protein is ATGP3368-010.

Q: Is Recombinant rat Lactate Dehydrogenase A/LDHA protein in stock?

Recombinant rat Lactate Dehydrogenase A/LDHA protein is currently made to order / not held in immediate stock. Contact us for lead time.

Ldha, also known as lactate dehydrogenase A, catalyzes the conversion of pyruvate to lactate, utilizing NADH as a cofactor. It has been identified as a potential therapeutic target in the area of cancer metabolism. Reduction in Ldha activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. Recombinant rat Ldha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Product Name Alternative

LDH muscle subunit, LDH-M

Expression System

Baculovirus

Antigen Species

Rat

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 200unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Molecular Weight

37.5 kDa (340aa)

Additionnal Information

Lactate Dehydrogenase A, LDHA, L-lactate dehydrogenase A chain, Ldha, Ldh1, ATGP3368-10 µg, ATGP3368-20 µg, ATGP3368-50 µg, ATGP3368-100 µg, ATGP3368-250 µg, ATGP3368-500 µg, ATGP3368-1 mg, ATGP3368-10, ATGP3368-20, ATGP3368-50, ATGP3368-100, ATGP3368-250, ATGP3368-500, ATGP3368-1

References & Citations

Ward RA., et al. (2012) J Med Chem. 55:3285-3306.; ; Fantin VR., et al. (2006) Cancer Cell. 9:425-434.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_058721

Uniprot Accession Number

P04642

Species

Rat

N Terminal Sequence

Met1

AA Sequence

MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH