Recombinant human TFPI-2 protein

TFPI2, as known as tissue factor pathway inhibitor 2 isoform 1, is a kunitz-type serine proteinase inhibitor, which is produced and secreted into endothelial cell matrix (ECM) by endothelial cells, smooth muscle cells, fibroblasts, keratinocytes, and urothelium. Also, this protein has been shown to inhibit ECM proteases essential for angiogenesis and metastasis. Recombinant human TFPI2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tissue factor pathway inhibitor 2 isoform 1, TFPI2, PP5, REF1, TFPI-2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.9 kDa (199aa)

Additionnal Information

TFPI2, Tissue factor pathway inhibitor 2 isoform 1, TFPI2, PP5, REF1, TFPI-2, ATGP3367-10 µg, ATGP3367-20 µg, ATGP3367-50 µg, ATGP3367-100 µg, ATGP3367-250 µg, ATGP3367-500 µg, ATGP3367-1 mg, ATGP3367-10, ATGP3367-20, ATGP3367-50, ATGP3367-100, ATGP3367-250, ATGP3367-500, ATGP3367-1

References & Citations

Arakawa N., et al. (2013) J. Proteome Res. 12:4340-4350.; ; Papareddy P., et al. (2012) PLoS ONE 7:E52772.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_006519

Uniprot Accession Number

P48307

Species

Human

N Terminal Sequence

Asp23

AA Sequence

DAAQEPTGNN AEICLLPLDY GPCRALLLRY YYDRYTQSCR QFLYGGCEGN ANNFYTWEAC DDACWRIEKV PKVCRLQVSV DDQCEGSTEK YFFNLSSMTC EKFFSGGCHR NRIENRFPDE ATCMGFCAPK KIPSFCYSPK DEGLCSANVT RYYFNPRYRT CDAFTYTGCG GNDNNFVSRE DCKRACAKAL KLEHHHHHH