Recombinant mouse Endoglin/CD105 protein
Eng, also known as endoglin, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. It is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. Recombinant mouse Eng, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse Endoglin (CAT# ATGP3361) is coated at 10 µg/mL (100 ul/well) can bind Human TGF-beta RI (CAT# ATGP4134) in a Functional ELISA assay.
Product Name Alternative
AI528660, AI662476, CD105, Cell surface MJ7/18 antigen, Endo, Endoglin, Eng, ORW, ORW1, Osler-Rendu-Weber syndrome 1, S-endoglin
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human TGF-beta RI (CAT# ATGP4134) .
Molecular Weight
60.9 kDa (563aa)
Additionnal Information
References & Citations
Ten Dijke P., et al. (2008) Angiogenesis. 11:79-89.; ; Bernabeu C., et al. (2007) J Cell Biochem. 102:1375-1388.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_031958
Uniprot Accession Number
Q63961
Species
Mouse
N Terminal Sequence
Glu27
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