Recombinant mouse Neuropilin 1/NRP1 protein

Nrp1, also known as Neuropilin 1, is a transmembrane glycoprotein that acts as a co-receptor for a number of extracellular ligands including class III/IV semaphorins, certain isoforms of vascular endothelial growth factor and transforming growth factor beta. It binds vascular endothelial growth factor (VEGF) -A and is thought to act as a coreceptor for kinase insert domain-containing receptor (KDR) by associating with KDR and enhancing VEGF signaling. It is a marker of regulatory T cells. Recombinant mouse Nrp1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nrp1, NPN-1, NP-1, Neuropilin-1, CD304, C530029I03, A5 protein

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

94.7 kDa (843aa)

Additionnal Information

Nrp-1, Nrp1, Nrp 1, Nrp, NPN-1, NPN1, NPN 1, NP-1, NP1, NP 1, Neuropilin-1, Neuropilin1, Neuropilin 1, CD304, CD 304, C530029I03, A5 protein, ATGP3360-10 µg, ATGP3360-20 µg, ATGP3360-50 µg, ATGP3360-100 µg, ATGP3360-250 µg, ATGP3360-500 µg, ATGP3360-1 mg, ATGP3360-010, ATGP3360-020, ATGP3360-050, ATGP3360-100, ATGP3360-250, ATGP3360-500, ATGP3360-01M

References & Citations

Chaudhary B., et al. (2014) Cancer Immunol Immunother. 63:81-99.; ; Herzoq B., et al. (2011) Mol Biol Cell. 22:2766-2776.; ; Glinka Y., et al. (2008) J Leukoc Biol. 84:302-310.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_032763

Uniprot Accession Number

P97333

Species

Mouse

N Terminal Sequence

Phe22

AA Sequence

FRSDKCGGTI KIENPGYLTS PGYPHSYHPS EKCEWLIQAP EPYQRIMINF NPHFDLEDRD CKYDYVEVID GENEGGRLWG KFCGKIAPSP VVSSGPFLFI KFVSDYETHG AGFSIRYEIF KRGPECSQNY TAPTGVIKSP GFPEKYPNSL ECTYIIFAPK MSEIILEFES FDLEQDSNPP GGMFCRYDRL EIWDGFPEVG PHIGRYCGQK TPGRIRSSSG VLSMVFYTDS AIAKEGFSAN YSVLQSSISE DFKCMEALGM ESGEIHSDQI TASSQYGTNW SVERSRLNYP ENGWTPGEDS YKEWIQVDLG LLRFVTAVGT QGAISKETKK KYYVKTYRVD ISSNGEDWIS LKEGNKAIIF QGNTNPTDVV LGVFSKPLIT RFVRIKPVSW ETGISMRFEV YGCKITDYPC SGMLGMVSGL ISDSQITASN QADRNWMPEN IRLVTSRTGW ALPPSPHPYT NEWLQVDLGD EKIVRGVIIQ GGKHRENKVF MRKFKIAYSN NGSDWKTIMD DSKRKAKSFE GNNNYDTPEL RTFSPLSTRF IRIYPERATH SGLGLRMELL GCEVEAPTAG PTTPNGNPVD ECDDDQANCH SGTGDDFQLT GGTTVLATEK PTIIDSTIQS EFPTYGFNCE FGWGSHKTFC HWEHDSHAQL RWSVLTSKTG PIQDHTGDGN FIYSQADENQ KGKVARLVSP VVYSQSSAHC MTFWYHMSGS HVGTLRVKLR YQKPEEYDQL VWMVVGHQGD HWKEGRVLLH KSLKLYQVIF EGEIGKGNLG GIAVDDISIN NHISQEDCAK PTDLDKKNTE IKIDETGSTP GYEGEGEGDK NISRKPGNVL KTLDPLEHHH HHH