Recombinant mouse Testican 3/SPOCK3 protein

Spock3, also known as testican-3, is a nervous system-expressed heparan sulfate proteoglycan belonging to a subgroup of the BM-40/SPARC/osteonectin family, the role of which in brain development is unclear. It inhibits the processing of pro-matrix metalloproteinase 2 (MMP-2) by MT1-MMP and MT3-MMP. It is mostly confined to the developmental stage of the brain. Recombinant mouse Spock3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Testican-3, Spock3, 2900045C01Rik, AI428471, mKIAA4039

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

47.9 kDa (423aa)

Additionnal Information

Testican3, SPOCK3, Testican-3, Spock3, 2900045C01Rik, AI428471, mKIAA4039, ATGP3359-10 µg, ATGP3359-20 µg, ATGP3359-50 µg, ATGP3359-100 µg, ATGP3359-250 µg, ATGP3359-500 µg, ATGP3359-1 mg, ATGP3359-10, ATGP3359-20, ATGP3359-50, ATGP3359-100, ATGP3359-250, ATGP3359-500, ATGP3359-1

References & Citations

Hartmann U., et al. (2013) J Neurochem. 125:399-409.; ; Yamamoto A., et al. (2014) Dev Neurosci. 36:381-395.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001239549

Uniprot Accession Number

Q8BKV0

Species

Mouse

N Terminal Sequence

Ala22

AA Sequence

AAAAVAVAGG RSDGGNFLDE KQWLTTISQY DKEVGQWNKF RDEVEDDYFR TWNPGKPFDQ ALDPAKDPCL KTKCSRHKVC ITQDAQTALC ISHRRLTHSM KEVGGSHKQW RGLPSSTCKP CPIAYASPVC GSDGHSYSSQ CKLEYQACVL GKQISIKCEG RCPCPSDKSM NIGRNVKRAC SDLEFREVAN RLRDWFKALH ESGSQNKKTK ALLRPERSRF DTSILPICKD SLGWMFNRLD TNYDLLLDQS ELGSIYLDKN EQCTKAFFNS CDTYKDSLIS NNEWCYCFQR QQDPPCHTEL SNIQKRQGIK KLLGQYIPLC DEDGYYKPTQ CHGSVGQCWC VDRYGNEVVG SRINGVADCA IDFEISGDFA SGDFREWTDD EGEEDDIMND KDDIEDDDED EGDDDDDGDV HDGYILEHHH HHH