Recombinant mouse Folate hydrolase 1/FOLH1 protein

Folh1, as known as glutamate carboxypeptidase 2, is a single pass type 2 membrane protein which belongs to the peptidase M28 family and M28B subfamily. This protein is most highly expressed in prostate epithelium. It is detected in urinary bladder, kidney, testis, ovary, live, stomach, small intestine colon, and the capillary endothelium of a variety of tumors. Thus, it shows a promising role in directed imaging and therapy of recurrent of metastatic disease. Recombinant mouse Folh1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Pteroylpoly-gamma-glutamate carboxypeptidase, Prostate-specific membrane antigen homolog, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Naalad1, Mopsm, mGCP, Membrane glutamate carboxypeptidase, Glutamate carboxypeptidase II, Glutamate carboxypeptidase 2, GCPII, Folylpoly-gamma-glutamate carboxypeptidase, FGCP

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

80.5 kDa (717aa)

Additionnal Information

Folh1, Glutamate carboxypeptidase 2 isoform 1, Folh1, GCP2, mopsm, ATGP3357-10 µg, ATGP3357-20 µg, ATGP3357-50 µg, ATGP3357-100 µg, ATGP3357-250 µg, ATGP3357-500 µg, ATGP3357-1 mg, ATGP3357-10, ATGP3357-20, ATGP3357-50, ATGP3357-100, ATGP3357-250, ATGP3357-500, ATGP3357-1

References & Citations

Rahn KA., et al, (2012) Proc. Natl. Acad. Sci. U.S.A. 109:20101-20106.; ; Schaevitz LR., et al, (2012) Dev Neurobiol 72:891-905.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_058050

Uniprot Accession Number

O35409

Species

Mouse

N Terminal Sequence

Ala

AA Sequence

ADPKPSNEAT GNVSHSGMKK EFLHELKAEN IKKFLYNFTR TPHLAGTQNN FELAKQIHDQ WKEFGLDLVE LSHYDVLLSY PNKTHPNYIS IINEDGNEIF KTSLSEQPPP GYENISDVVP PYSAFSPQGT PEGDLVYVNY ARTEDFFKLE REMKISCSGK IVIARYGKVF RGNMVKNAQL AGAKGMILYS DPADYFVPAV KSYPDGWNLP GGGVQRGNVL NLNGAGDPLT PGYPANEHAY RHELTNAVGL PSIPVHPIGY DDAQKLLEHM GGPAPPDSSW KGGLKVPYNV GPGFAGNFST QKVKMHIHSY TKVTRIYNVI GTLKGALEPD RYVILGGHRD AWVFGGIDPQ SGAAVVHEIV RSFGTLKKKG RRPRRTILFA SWDAEEFGLL GSTEWAEEHS RLLQERGVAY INADSSIEGN YTLRVDCTPL MYSLVYNLTK ELQSPDEGFE GKSLYDSWKE KSPSPEFIGM PRISKLGSGN DFEVFFQRLG IASGRARYTK NWKTNKVSSY PLYHSVYETY ELVVKFYDPT FKYHLTVAQV RGAMVFELAN SIVLPFDCQS YAVALKKYAD TIYNISMKHP QEMKAYMISF DSLFSAVNNF TDVASKFNQR LQELDKSNPI LLRIMNDQLM YLERAFIDPL GLPGRPFYRH IIYAPSSHNK YAGESFPGIY DALFDISSKV NASKAWNEVK RQISIATFTV QAAAETLREV AHHHHHH