Recombinant human Protein O-glucosyltransferase 1/POGLUT1 protein

POGLUT1, also known as protein O-glucosyltransferase 1, is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER) -retaining glucosyltransferase that adds glucose to serine residues within the consensus sequence of C1-X-S-X-P-C2 in Notch EGF repeats, thereby regulating cell-fate decisions. Recombinant human POGLUT1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CAP10-like 46 kDa protein, hCLP46, KTEL (Lys-Tyr-Glu-Leu) containing 1, Myelodysplastic syndromes relative protein, O-glucosyltransferase Rumi homolog, C3orf9, KDELC family like 1, KTELC1, MDSRP, MDS010

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.5 kDa (377aa)

Additionnal Information

C3orf9, CAP10 like 46 kDa protein, CAP10 like 46kDa protein, CAP10-like 46 kDa protein, CAP10-like 46kDa protein, CLP 46, CLP46, hCLP 46, hCLP46, KDELC family like 1, KDELCL 1, KDELCL1, KTEL (Lys Tyr Glu Leu) containing 1, KTEL (Lys-Tyr-Glu-Leu) containing 1, KTEL containing 1, KTELC 1, KTELC1, MDS010, MDSRP, Myelodysplastic syndromes relative protein, O glucosyltransferase Rumi homolog, O-glucosyltransferase Rumi homolog, POGLUT1, Protein O glucosyltransferase 1, Protein O-glucosyltransferase 1, ATGP3356-10 µg, ATGP3356-20 µg, ATGP3356-50 µg, ATGP3356-100 µg, ATGP3356-250 µg, ATGP3356-500 µg, ATGP3356-1 mg, ATGP3356-010, ATGP3356-020, ATGP3356-050, ATGP3356-100, ATGP3356-250, ATGP3356-500, ATGP3356-01M

References & Citations

Acar M., et al. (2008) Cell 132:247-258.; ; Teng Y., et al. (2006) Gene. 371:7-15.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Development

NCBI Accession Number

NP_689518

Uniprot Accession Number

Q8NBL1

Species

Human

N Terminal Sequence

Arg24

AA Sequence

RQKESGSKWK VFIDQINRSL ENYEPCSSQN CSCYHGVIEE DLTPFRGGIS RKMMAEVVRR KLGTHYQITK NRLYRENDCM FPSRCSGVEH FILEVIGRLP DMEMVINVRD YPQVPKWMEP AIPVFSFSKT SEYHDIMYPA WTFWEGGPAV WPIYPTGLGR WDLFREDLVR SAAQWPWKKK NSTAYFRGSR TSPERDPLIL LSRKNPKLVD AEYTKNQAWK SMKDTLGKPA AKDVHLVDHC KYKYLFNFRG VAASFRFKHL FLCGSLVFHV GDEWLEFFYP QLKPWVHYIP VKTDLSNVQE LLQFVKANDD VAQEIAERGS QFIRNHLQMD DITCYWENLL SEYSKFLSYN VTRRKGYDQI IPKMLKTELL EHHHHHH