Recombinant E.coli Maltose Binding Protein/MBP protein

MBP also as known as Maltose Binding Protein is a protein related with the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has been used to increase the yield of its fusion partner in many cases. In addition, MBP is often able to promote the solubility of polypeptides to which it is fused. Recombinant MBP was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

ZCSL3, MMBP, MBP, Maltose binding protein, Maltodextrin-binding protein, malJ, malE, JW3994, JJJ3, Escherichia coli MBP, ECK4026, E.coli MBP, DPH4, Cytoplasmic maltose-binding protein

Expression System

E.coli

Antigen Species

E.coli

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

40.8 kDa (371aa)

Additionnal Information

MBP, ECK4026, JW3994, malJ, ATGP3346-10 µg, ATGP3346-20 µg, ATGP3346-50 µg, ATGP3346-100 µg, ATGP3346-250 µg, ATGP3346-500 µg, ATGP3346-1 mg, ATGP3346-10, ATGP3346-20, ATGP3346-50, ATGP3346-100, ATGP3346-250, ATGP3346-500, ATGP3346-1

References & Citations

Fox JD., et al. (2001) Protein Sci. 10 (3) : 622-30.; ; Riggs P., et al. (2000) Mol. Biotechnol. 15 (1) : 51-63.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Fusion Tag & Detection

NCBI Accession Number

NP_418458.1

Uniprot Accession Number

P0AEX9

Species

E.coli

AA Sequence

MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTRIT K