Recombinant mouse c-kit Ligand/SCF protein

Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. SCF binds to and activates the SCF receptor (SCFR), a receptor tyrosine kinase. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell-cell adhesion and acts synergistically with other cytokine. Recombinant mouse SCF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Specifications

Mouse SCF/c-kit Ligand stimulates cell proliferation of the MC/9 mouse mast cells. The ED50 range ≤7 ng/mL.

Product Name Alternative

Kit ligand, KITLG, Mast cell growth factor, MGF, Stem cell factor, SCF, c-Kit ligand, Steel factor, SF, Kitl, Hematopoietic growth factor KL, KL-1, Familial progressive hyperpigmentation 2, FPH2, SLF, blz, Gb, Grizzle-belly, SL

Expression System

E.coli

Antigen Species

Mouse

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 range ≤7 ng/mL.

Molecular Weight

18 kDa (165aa) confirmed by MALDI-TOF

Additionnal Information

SCF, c-kit Ligand, ATGP3345-10 µg, ATGP3345-20 µg, ATGP3345-50 µg, ATGP3345-100 µg, ATGP3345-250 µg, ATGP3345-500 µg, ATGP3345-1 mg, ATGP3345-10, ATGP3345-20, ATGP3345-50, ATGP3345-100, ATGP3345-250, ATGP3345-500, ATGP3345-1

References & Citations

Zhang Z., et al. (2000) . Proc. Natl. Acad. Sci. U.S.A. 97, 7732.; ; Okada S , et al. (1992) . Nippon Rinsho , 50, 1872.

Other References

Yamada T, et al. Melanocyte stem cells express receptors for canonical Wnt-signaling pathway on their surface. (Biochem Biophys Res Commun. 2010) {https://pubmed.ncbi.nlm.nih.gov/20450888/}; ; Hattori K, et al. Immunostimulatory effects of a subcritical water extract of Ganoderma. (Biomed Rep. 2022) {https://pubmed.ncbi.nlm.nih.gov/36544853/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_038626

Uniprot Accession Number

P20826

Species

Mouse

AA Sequence

MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA