Recombinant human CD30 Ligand/TNFSF8 protein

TNFSF8, also known as tumor necrosis factor ligand superfamily member 8, is a type II membrane protein belonging to the TNF superfamily. It is expressed on the cell surface of activated T cells, B cells, and monocytes. CD30/TNFRSF8 ligation by TNFSF8 mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis. Recombinant human TNFSF8, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CD30 Ligand/TNFSF8 is coated at 2 µg/mL (100 ul/well) can bind Human CD30/TNFRSF8 (CAT# ATGP3389) in a Functional ELISA assay.

Product Name Alternative

Tumor necrosis factor ligand superfamily member 8, TNFSF8, CD153, CD30L, CD30LG, TNLG3A

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human CD30/TNFRSF8 (CAT# ATGP3389)

Molecular Weight

20.7 kDa (181aa)

Additionnal Information

CD30 Ligand, TNFSF8, Tumor necrosis factor ligand superfamily member 8, TNFSF8, CD153, CD30L, CD30LG, TNLG3A, ATGP3343-10 µg, ATGP3343-20 µg, ATGP3343-50 µg, ATGP3343-100 µg, ATGP3343-250 µg, ATGP3343-500 µg, ATGP3343-1 mg, ATGP3343-10, ATGP3343-20, ATGP3343-50, ATGP3343-100, ATGP3343-250, ATGP3343-500, ATGP3343-1

References & Citations

Duckett CS., et al. (1997) Mol. Cell. Biol. 17:1535-1542.; ; Chiarle R., et al. (1999) J. Immunol. 163:194-205.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001235

Uniprot Accession Number

P32971

Species

Human

N Terminal Sequence

Ala

AA Sequence

QRTDSIP NSPDNVPLKG GNCSEDLLCI LKRAPFKKSW AYLQVAKHLN KTKLSWNKDG ILHGVRYQDG NLVIQFPGLY FIICQLQFLV QCPNNSVDLK LELLINKHIK KQALVTVCES GMQTKHVYQN LSQFLLDYLQ VNTTISVNVD TFQYIDTSTF PLENVLSIFL YSNSD