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Recombinant mouse Peroxiredoxin 1/PRDX1 protein

Prdx1, also known as peroxiredoxin-1, is an important member of peroxiredoxins (Prdxs) regulating various cellular signaling and differentiation. It confers an aggressive survival phenotype of cancer cells and drug-resistance. This protein is also identified as a red blood cell factor which enhances natural killer (NK) cell activity. Recombinant mouse Prdx1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >2,500 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.

Product Name Alternative

Macrophage 23kDa stress protein, Macrophase stress protein 22kDa, MSP23, OSF-3, Osteoblast specific factor 3, PAG, Paga, prx1, PrxI, Prx I, Tdpx2, TDX2, Thioredoxin dependent peroxide reductase 2, Trx dependent peroxide reductase 2

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is >4,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.

Molecular Weight

23.2 kDa (207aa)

Additionnal Information

Peroxiredoxin1, Prdx1, Peroxiredoxin-1, Prdx1, MSP23, NkefA, OSF-3, OSF3, PAG, Paga, Prdx I, prdx1, Prx I, Tdpx2, TDX2, TPxA, ATGP3340-10 µg, ATGP3340-20 µg, ATGP3340-50 µg, ATGP3340-100 µg, ATGP3340-250 µg, ATGP3340-500 µg, ATGP3340-1 mg, ATGP3340-10, ATGP3340-20, ATGP3340-50, ATGP3340-100, ATGP3340-250, ATGP3340-500, ATGP3340-1

References & Citations

Zhou J., et al. (2015) Int J Clin Exp Pathol. 8:9863-9874.; ; Sauri H., et al. (1996) J Leukoc Biol. 59:925-931.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_035164

Uniprot Accession Number

P35700

Species

Mouse

N Terminal Sequence

Met1

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