Recombinant mouse Peroxiredoxin 1/PRDX1 protein
Prdx1, also known as peroxiredoxin-1, is an important member of peroxiredoxins (Prdxs) regulating various cellular signaling and differentiation. It confers an aggressive survival phenotype of cancer cells and drug-resistance. This protein is also identified as a red blood cell factor which enhances natural killer (NK) cell activity. Recombinant mouse Prdx1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is >2,500 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is >4,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.
Molecular Weight
23.2 kDa (207aa)
Additionnal Information
References & Citations
Zhou J., et al. (2015) Int J Clin Exp Pathol. 8:9863-9874.; ; Sauri H., et al. (1996) J Leukoc Biol. 59:925-931.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_035164
Uniprot Accession Number
P35700
Species
Mouse
N Terminal Sequence
Met1
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