Recombinant human ST6GAL1 protein

ST6GAL1, also known as beta-galactoside alpha-2, 6-sialyltransferase 1, is a type II membrane protein localized in the trans-Golgi network and catalyzes 2, 6-sialylation of Gal beta 1, 4-GlcNAc structures on N-glycans. It is highly expressed in the liver and also expressed in most other tissues to some extent. Its deficiency causes abnormalities in B cell immunoreactivity. Recombinant human ST6GAL1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Beta-galactoside alpha-2,6-sialyltransferase 1, ST6GAL1, SIAT1, ST6GalI, ST6N

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.6 kDa (389aa)

Additionnal Information

ST6GAL1, Beta-galactoside alpha-2,6-sialyltransferase 1, ST6GAL1, SIAT1, ST6GalI, ST6N, ATGP3338-10 µg, ATGP3338-20 µg, ATGP3338-50 µg, ATGP3338-100 µg, ATGP3338-250 µg, ATGP3338-500 µg, ATGP3338-1 mg, ATGP3338-10, ATGP3338-20, ATGP3338-50, ATGP3338-100, ATGP3338-250, ATGP3338-500, ATGP3338-1

References & Citations

Kuhn B., et al. (2013) Acta Crystallogr D Biol Crystallogr. 69:1826-1838.; ; Wu ZL., et al. (2011) Glycobiology. 21:727-733.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_775323

Uniprot Accession Number

P15907

Species

Human

N Terminal Sequence

Ala

AA Sequence

KEKKKGS YYDSFKLQTK EFQVLKSLGK LAMGSDSQSV SSSSTQDPHR GRQTLGSLRG LAKAKPEASF QVWNKDSSSK NLIPRLQKIW KNYLSMNKYK VSYKGPGPGI KFSAEALRCH LRDHVNVSMV EVTDFPFNTS EWEGYLPKES IRTKAGPWGR CAVVSSAGSL KSSQLGREID DHDAVLRFNG APTANFQQDV GTKTTIRLMN SQLVTTEKRF LKDSLYNEGI LIVWDPSVYH SDIPKWYQNP DYNFFNNYKT YRKLHPNQPF YILKPQMPWE LWDILQEISP EEIQPNPPSS GMLGIIIMMT LCDQVDIYEF LPSKRKTDVC YYYQKFFDSA CTMGAYHPLL YEKNLVKHLN QGTDEDIYLL GKATLPGFRT IHC