Recombinant human CD27 Ligand/CD70 protein
CD70, also known as CD70 antigen, is a transmembrane protein in the TNF receptor superfamily. It functions as a co-stimulatory molecule that supports lymphocyte activation and survival. This protein binds to the transmembrane glycoprotein CD27 ligand/CD70 which is expressed on activated B cells, activated T cells, and dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T cell activation, and thus is proliferation of co-stimulated T cells, as well as the generation of cytolytic T cells. Recombinant human CD70, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human CD27 Ligand/TNFSF7 is coated at 10 µg/mL (100 ul/well) can bind Human CD27/TNFRSF7 (CAT# ATGP3416) in a Functional ELISA assay.
Product Name Alternative
CD70 antigen, CD27L, CD27LG, TNFSF7, tumor necrosis factor superfamily member 7
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human CD27/TNFRSF7 (CAT# ATGP3416) .
Molecular Weight
18.2 kDa (164aa)
Additionnal Information
References & Citations
Welten SP., et al. (2013) J. Virol. 87:6851-6865.; ; Polak ME., et al. (2012) J. Invest. Dermatol. 132:1636-1644.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001243.1
Uniprot Accession Number
P32970
Species
Human
N Terminal Sequence
Ala
AA Sequence
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