Recombinant human EGFR protein
EGFR, also known as epidermal growth factor receptor, is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinase. This protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-alpha, betacellulin, etc. It can also be recruited to form heterodimers with the ligand activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. It is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs. Recombinant human EGFR, fused to hIgG-His-tag at C-terminus, was expressed in CHO cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
CHO Cell
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
95.5 kDa (860aa)
Additionnal Information
References & Citations
Schlessinger J., et al. (200) Cell. 103:211-225.; ; Singh A.B., et al. (2005) Cell. Signal. 17:1183-1193.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_005219
Uniprot Accession Number
P00533
Species
Human
N Terminal Sequence
Leu25
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
