Recombinant human TPST1 protein

TPST1, also known as protein-tyrosine sulfotransferase 1, is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins. It utilizes 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfonate donor and binds proteins with target tyrosine residues to eventually form the tyrosine O-sulfate ester group and the desulfonated 3'-phosphoadenosine-5'-phosphate (PAP) . Recombinant human TPST1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein-tyrosine sulfotransferase 1, TANGO13A

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

40.6 kDa (354aa)

Additionnal Information

TPST1, Protein-tyrosine sulfotransferase 1, TANGO13A, ATGP3331-10 µg, ATGP3331-20 µg, ATGP3331-50 µg, ATGP3331-100 µg, ATGP3331-250 µg, ATGP3331-500 µg, ATGP3331-1 mg, ATGP3331-10, ATGP3331-20, ATGP3331-50, ATGP3331-100, ATGP3331-250, ATGP3331-500, ATGP3331-1

References & Citations

Ouyang Y.B., et al. (1998) Proc Natl Acad Sci USA. 95:2896-2901.; ; Danan L.M., et al. (2008) J. Am. Soc. Mass. Spectrom. 19:1459-1466.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_003587

Uniprot Accession Number

O60507

Species

Human

N Terminal Sequence

Ala

AA Sequence

QHAMECH HRIEERSQPV KLESTRTTVR TGLDLKANKT FAYHKDMPLI FIGGVPRSGT TLMRAMLDAH PDIRCGEETR VIPRILALKQ MWSRSSKEKI RLDEAGVTDE VLDSAMQAFL LEIIVKHGEP APYLCNKDPF ALKSLTYLSR LFPNAKFLLM VRDGRASVHS MISRKVTIAG FDLNSYRDCL TKWNRAIETM YNQCMEVGYK KCMLVHYEQL VLHPERWMRT LLKFLQIPWN HSVLHHEEMI GKAGGVSLSK VERSTDQVIK PVNVGALSKW VGKIPPDVLQ DMAVIAPMLA KLGYDPYANP PNYGKPDPKI IENTRRVYKG EFQLPDFLKE KPQTEQVE