Recombinant human TPST1 protein
TPST1, also known as protein-tyrosine sulfotransferase 1, is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins. It utilizes 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfonate donor and binds proteins with target tyrosine residues to eventually form the tyrosine O-sulfate ester group and the desulfonated 3'-phosphoadenosine-5'-phosphate (PAP) . Recombinant human TPST1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Protein-tyrosine sulfotransferase 1, TANGO13A
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
40.6 kDa (354aa)
Additionnal Information
References & Citations
Ouyang Y.B., et al. (1998) Proc Natl Acad Sci USA. 95:2896-2901.; ; Danan L.M., et al. (2008) J. Am. Soc. Mass. Spectrom. 19:1459-1466.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cardiovascular
NCBI Accession Number
NP_003587
Uniprot Accession Number
O60507
Species
Human
N Terminal Sequence
Ala
AA Sequence
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