Recombinant mouse Meprin beta Subunit/MEP1B protein

MEP1B, also known as meprin A subunit beta, is a member of the astacin family of zinc endopeptidases 1, 2. It is disulfide-linked, tetrameric metalloendopeptidases in renal brush border membranes. It is highly regulated, secreted, and cell-surface metalloendopeptidases that are abundantly expressed in the kidney and intestine. Recombinant mouse MEP1B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Meprin A subunit beta, Mep1b, Mep-1b

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

72.6 kDa (642aa)

Additionnal Information

Meprin b Subunit, MEP1B, Meprin A subunit beta, Mep1b, Mep-1b, ATGP3323-10 µg, ATGP3323-20 µg, ATGP3323-50 µg, ATGP3323-100 µg, ATGP3323-250 µg, ATGP3323-500 µg, ATGP3323-1 mg, ATGP3323-10, ATGP3323-20, ATGP3323-50, ATGP3323-100, ATGP3323-250, ATGP3323-500, ATGP3323-1

References & Citations

Bertenshaw GP., et al. (2001) J Biol Chem. 276:13248-13255.; ; Kounnas MZ., et al. (1991) J Biol Chem. 266:17350-17357.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_032612

Uniprot Accession Number

Q61847

Species

Mouse

N Terminal Sequence

Leu21

AA Sequence

LPAPEKFVKD IDGGIDQDIF DINQGLGLDL FEGDIKLEAN GKNSIIGDHK RWPHTIPYVL EDSLEMNAKG VILNAFERYR LKTCIDFKPW SGEANYISVF KGSGCWSSVG NIHAGKQELS IGTNCDRIAT VQHEFLHALG FWHEQSRADR DDYVIIVWDR IQPGKEHNFN IYNDSVSDSL NVPYDYTSVM HYSKTAFQNG TESTIVTRIS EFEDVIGQRM DFSDYDLLKL NQLYNCTSSL SFMDSCDFEL ENICGMIQSS GDSADWQRVS QVLSGPESDH SKMGQCKDSG FFMHFNTSIL NEGATAMLES RLLYPKRGFQ CLEFYLYNSG SGNDQLNIYT REYTTGQQGG VLTLQRQIKE VPIGSWQLHY VTLQVTKKFR VVFEGLRGPG TSSGGLSIDD INLSETRCPH HIWHIQNFTQ ILGGQDTSVY SPPFYSSKGY AFQIYMDLRS STNVGIYFHL ISGANDDQLQ WPCPWQQATM TLLDQNPDIR QRMFNQRSIT TDPTMTSDNG SYFWDRPSKV GVTDVFPNGT QFSRGIGYGT TVFITRERLK SREFIKGDDI YILLTVEDIS HLNSTSAVPD PVPTLAVHNA CSEVVCQNGG ICVVQDGRAE CKCPAGEDWW YMGKRCEKRG STRDVEHHHH HH