Recombinant human PTH protein
Human parathyroid hormone (hPTH) is an 84 amino acid residue peptide and one of the main regulators in maintenance of calcium homeostasis. It acts primarily on kidney and bone cells stimulating calcium back resorption or calcium mobilization, respectively. The whole mechanism is still under discussion, but low-dose hPTH triggers cyclic AMP-dependent protein kinase in some populations of bone cells bearing PTH receptors, which stimulates the proliferation of osteoblasts. Human parathyroid hormone (amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques
Product Specifications
Specifications
Human PTH Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 range ≤ 50 ng/mL.
Product Name Alternative
PTH, PTH1
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 range ≤ 50 ng/mL.
Molecular Weight
9.55 kDa (85aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hodsman AB. et al. (2003) J. Clin. Endocrinol. Metab. 88 (11), 5212-20.; ; Wingender E., et al. (1989) J. Biol. Chem. 264 (8),4367-73.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 2mM EDTA
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000306
Uniprot Accession Number
P01270
Species
Human
AA Sequence
MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ
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