Recombinant human CNTF protein

CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM) . It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CNTF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤500 ng/mL.

Product Name Alternative

Ciliary neurotrophic factor

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤500 ng/mL.

Molecular Weight

22.9 kDa (200aa) confirmed by MALDI-TOF

Additionnal Information

CNTF, ATGP3316-10 µg, ATGP3316-20 µg, ATGP3316-50 µg, ATGP3316-100 µg, ATGP3316-250 µg, ATGP3316-500 µg, ATGP3316-1 mg, ATGP3316-10, ATGP3316-20, ATGP3316-50, ATGP3316-100, ATGP3316-250, ATGP3316-500, ATGP3316-1

References & Citations

Sendther M., et al. (1994) J Neurobiol. 25 (11) :1436-53.; ; Robert F., et al. (2007) J Biol Chem. 282 (46) :33421-33434.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000605

Uniprot Accession Number

P26441

Species

Human

AA Sequence

MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM