Recombinant human CNTF protein
CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM) . It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human CNTF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤500 ng/mL.
Product Name Alternative
Ciliary neurotrophic factor
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤500 ng/mL.
Molecular Weight
22.9 kDa (200aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Sendther M., et al. (1994) J Neurobiol. 25 (11) :1436-53.; ; Robert F., et al. (2007) J Biol Chem. 282 (46) :33421-33434.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000605
Uniprot Accession Number
P26441
Species
Human
AA Sequence
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