Recombinant human CNTF protein

Human CNTF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤500 ng/mL.

Ciliary neurotrophic factor

E.coli

Human

Non-Tagged

SDS-PAGE, Bioactivity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤500 ng/mL.

22.9 kDa (200aa) confirmed by MALDI-TOF

Sendther M., et al. (1994) J Neurobiol. 25 (11) :1436-53.; ; Robert F., et al. (2007) J Biol Chem. 282 (46) :33421-33434.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Hormones & Cytokines

NP_000605

P26441

Human