Recombinant rat Cystatin C protein

CST3, also known as cystain-C, is secreted type 2 cysteine proteinase inhibitors of the cystatin superfamily. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S. Recombinant rat CST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

The IC50 value is < 1.0 nm. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Product Name Alternative

Cystain-C, CST3, CYSC

Expression System

Baculovirus

Antigen Species

Rat

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

The IC50 value is < 1.0 nm. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Molecular Weight

14.3 kDa (128aa)

Additionnal Information

Cystatin C, Cystain-C, CST3, CYSC, ATGP3313-10 µg, ATGP3313-20 µg, ATGP3313-50 µg, ATGP3313-100 µg, ATGP3313-250 µg, ATGP3313-500 µg, ATGP3313-1 mg, ATGP3313-10, ATGP3313-20, ATGP3313-50, ATGP3313-100, ATGP3313-250, ATGP3313-500, ATGP3313-1