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Recombinant rat Cystatin C protein

CST3, also known as cystain-C, is secreted type 2 cysteine proteinase inhibitors of the cystatin superfamily. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S. Recombinant rat CST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

The IC50 value is < 1.0 nm. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Product Name Alternative

Cystain-C, CST3, CYSC

Expression System

Baculovirus

Antigen Species

Rat

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

The IC50 value is < 1.0 nm. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Molecular Weight

14.3 kDa (128aa)

Additionnal Information

Cystatin C, Cystain-C, CST3, CYSC, ATGP3313-10 µg, ATGP3313-20 µg, ATGP3313-50 µg, ATGP3313-100 µg, ATGP3313-250 µg, ATGP3313-500 µg, ATGP3313-1 mg, ATGP3313-10, ATGP3313-20, ATGP3313-50, ATGP3313-100, ATGP3313-250, ATGP3313-500, ATGP3313-1

References & Citations

Reed CH., et al. (2000) Br J Biomed. Sci. 57:323-329.; ; Esnard A., et al. (1992) FEBS Lett. 300:131-135.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_036969

Uniprot Accession Number

P14841

Species

Rat

N Terminal Sequence

Gly21

AA Sequence

GTSRPPPRLL GAPQEADASE EGVQRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGI NYYLDVEMGR TTCTKSQTNL TNCPFHDQPH LMRKALCSFQ IYSVPWKGTH TLTKSSCKNA LEHHHHHH

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